Publikationen

2020      
  Gao Y, Wang L, Kalscheuer R, Liu Z, Proksch P. Antifungal polyketide derivatives from the endophytic fungus Aplosporella javeedii. Bioorg Med Chem 28, 115456 (2020).    
  Moussa M, Ebrahim W, Kalscheuer R,  Liu Z, Proksch P. Co-culture of the bacterium Pseudomonas aeruginosa with the fungus Fusarium tricinctum induces bacterial antifungal and quorum sensing signaling molecules. Phytochem Lett 36, 37-41 (2020).    
  Yu X, Müller W, Meier D, Kalscheuer R, Guo Z, Zou K, Umeokoli B, Liu Z, Proksch P. Polyketide derivatives from mangrove derived endophytic fungus Pseudopestalotiopsis theae. Mar Drugs 18, 129 (2020).    
  Javid A, Cooper C, Singh A, Schindler S, Hänisch M, Marshall RL, Kalscheuer R, Bavro VN, Bhatt A. The mycolic acid reductase Rv2509 has distinct structural motifs and is essential for growth in slow-growing mycobacteria. Mol Microbiol 113, 521-533 (2020).    
  Pan F, EI-Kashef D, Kalscheuer R, Müller WEG, Lee J, Feldbrügge M, Mándi A, Kurtán T, Liu Z, Wu W, Proksch P. Cladosins L-O, new hybrid polyketides from the endophytic fungus Cladosporium sphaerospermum WBS017. Eur J Med Chem 191, 112159 (2020).    
  Ariantari N, Ancheeva E, Frank M, Stuhldreier F, Meier D, Gröner Y, Reimche I, Teusch N, Wesselborg S, Müller W, Kalscheuer R, Liu Z, Proksch P. Didymellanosine, a new decahydrofluorene analogue, and ascolactone C from Didymella sp. IEA-3B.1, an endophyte of Terminalia catappa. RSC Advances 10:7232–7240 (2020).    
  Schages L, Wichern F, Kalscheuer R, Bockmühl D. Winter is coming – Impact of temperature on the variation of beta-lactamase and mcr genes in a wastewater treatment plant. Sci Total Environ 712:136499 (2020).    
2019      
  Meier D, Vázquez Hernández M, van Geelen L, Muharini R, Proksch P, Bandow JE, Kalscheuer R. The plant-derived chalcone Xanthoangelol targets the membrane of Gram-positive bacteria. Bioorg Med Chem 27, 115151 (2019).    
  Lienau C, Gräwert T, Alves Avelar LA, Illarionov B, Held J, Knaab TC, Lungerich B, van Geelen L, Meier D, Geissler S, Cynis H, Riederer U, Buchholz M, Kalscheuer R, Bacher A, Mordmüller B, Fischer M, Kurz T. Novel reverse thia-analogs of fosmidomycin: Synthesis and antiplasmodial activity. Eur J Med Chem 181, 11555 (2019).    
  Tran-Cong NM, Mándi A, Kurtán T, Müller WEG, Kalscheuer R, Lin W, Liu Z, Proksch P. Induction of cryptic metabolites of the endophytic fungus Trichocladium sp. through OSMAC and co-cultivation. RSC Advance 9, 27279–27288 (2019).    
  Rehberg N, Omeje E, Ebada SS, van Geelen L, Liu Z, Sureechatchayan P, Kassack MU, Ioerger TR, Proksch P, Kalscheuer R. 3-O-Methyl-alkylgallates inhibit fatty acid desaturation in Mycobacterium tuberculosis. Antimicrob Agents Chemother 63, e00136-19 (2019).    
  Steindor M, Nkwouano V, Stefanski A, Stuehler K, Ioerger TR, Bogumil D, Jacobsen M, Mackenzie CR, Kalscheuer R. A proteomics approach for the identification of species-specific immunogenic proteins in the Mycobacterium abscessus complex. Microbes Infect 21, 154-162 (2019).    
  Kalscheuer R, Palacios A, Anso I, Cifuente J, Anguita J, Jacobs WR, Jr., Guerin ME, Prados-Rosales R. The Mycobacterium tuberculosis capsule: a cell structure with key implications in pathogenesis. Biochem J 476, 1995-2016 (2019).    
  Zaschke-Kriesche J, Behrmann LV, Reiners J, Lagedroste M, Gröner Y, Kalscheuer R, Smits SHJ. Bypassing lantibiotic resistance by an effective nisin derivative. Bioorg Med Chem 27, 3454-3462 (2019).    
  Moussa M, Weaam E, Bonus M, Gohlke H, Mándi A, Kurtán T, Hartmann R, Kalscheuer R, Lin W, Liu Z, Proksch P. Co-culture of the fungus Fusarium tricinctum with Streptomyces lividans induces production of cryptic naphthoquinone dimers. RSC Advances 9, 1491-1500 (2019).    
  Abdel-Wahab NM, Scharf S, Özkaya FC, Kurtan T, Mandi A, Fouad MA, Kamel MS, Müller WEG, Kalscheuer R, Lin WH, Daletos G, Ebrahim W, Liu Z, Proksch P. Induction of Secondary Metabolites from the Marine-Derived Fungus Aspergillus versicolor through Co-cultivation with Bacillus subtilis. Planta Med 85, 503-512 (2019).    
  Umeokoli BO, Ebrahim W, El-Neketi M, Muller WEG, Kalscheuer R, Lin W, Liu Z, Proksch P. A new depsidone derivative from mangrove sediment derived fungus Lasiodiplodia theobromae. Nat Prod Res 33, 2215-2222 (2019).    
2018      
  Özkaya FC, Ebrahim W,  El-Neketi M,  Tansel Tanrikul T,  Kalscheuer R,  Müller WEG, Guo Z, Zou K, Liu Z, Proksch P. Induction of new metabolites from sponge-associated fungus Aspergillus carneus by OSMAC approach. Fitoterapia 131, 9–14. doi: 10.1016/j.fitote.2018.10.008 (2018).    
  Abdelwahab MF, Fouad MA, Kamel MS, Özkaya FC, Kalscheuer R, Müller WEG, Lin W, Liu Z, Ebrahim W, Daletos G, Proksch P. Tanzawaic acid derivatives from freshwater sediment-derived fungus Penicillium sp. Fitoterapia 128, 258-264. doi: 10.1016/j.fitote.2018.05.019 (2018).    
  Kamdem RST, Pascal W, Rehberg N, van Geelen L, Höfert SP, Knedel TO, Janiak C, Sureechatchaiyan P, Kassack MU, Lin W, Kalscheuer R, Liu Z, Proksch P. Metabolites from the endophytic fungus Cylindrocarpon sp. isolated from tropical plant Sapium ellipticum. Fitoterapia 128, 175-179. doi: 10.1016/j.fitote.2018.05.020 (2018).    
  Kamariza M, Shieh P, Ealand CS, Peters JS, Chu B, Rodriguez-Rivera FP, Babu Sait MR, Treuren WV, Martinson N, Kalscheuer R, Kana BD, Bertozzi CR. Rapid detection of Mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe. Sci Translat Med 10, eaam6310  (2018).    
  van Geelen L, Meier D, Rehberg N, Kalscheuer R. (Some) current concepts in antibacterial drug discovery. Appl Microbiol Biotechnol 102, 2949-2963 (2018).    
  Rehberg N, Akone HS, Ioerger TR, Erlenkamp G, Daletos G, Gohlke H, Proksch P, Kalscheuer R. Chlorflavonin targets acetohydroxyacid synthase catalytic subunit IlvB1 for synergistic killing of Mycobacterium tuberculosis. ACS Infect Dis. 2018 Feb 9;4(2):123-134. doi: 10.1021/acsinfecdis.7b00055. Epub 2017 Nov 16.    
  Ebada SS, El-Neketi M, Ebrahim W, Mándi A, Kurtán T, Kalscheuer R, Müller WEG, Proksch P. Cytotoxic secondary metabolites from the endophytic fungus Aspergillus versicolor KU258497. Phytochem Lett 24, 88-93. 10.1016/j.phytol.2018.01.010 (2018).    
2017      
  Marmouzi I, Faouzi, ME, Saidi N, Cherrah Y, Rehberg N, Ebada SS, Ebrahim W, Kalscheuer R, Proksch P. Bioactive secondary metabolites from Chaetomium globosum, an endophyte from the Moroccan plant Avena sativa. ‎Chem Nat Compd 53, 1208-1211. doi: 10.1007/s10600-017-2242-6 (2017).    
  Mokhlesi A, Stuhldreier F, Wex KW, Berscheid A, Hartmann R, Rehberg N, Sureechatchaiyan P, Chaidir C, Kassack MU, Kalscheuer R, Brötz-Oesterhelt H, Wesselborg S, Stork B, Daletos G, Proksch P. Cyclic cystine-bridged peptides from the marine sponge Clathria basilana induce apoptosis in tumor cells and depolarize the bacterial cytoplasmic membrane. J Nat Prod 80, 2941-2952. doi: 10.1021/acs.jnatprod.7b00477 (2017).    
  Umeokoli BO, Onyegbule FA, Okoye FBC, Wang H, Kalscheuer R, Müller WEG, Hartmann R, Liu Z, Proksch P. New amide and dioxopiperazine derivatives from leaves of Breynia nivosa. Fitoterapia 122, 16-19. doi: 10.1016/j.fitote.2017.08.009 (2017).    
  Ebada SS, de Voogd N, Kalscheuer R, Mueller WEG, Chaidir, Proksch P. Cytotoxic drimane meroterpenoids from the Indonesian marine sponge Dactylospongia elegans. Phytochem Lett 22, 154-158. doi: 10.1016/j.phytol.2017.09.026 (2017).    
  Elnaggar MS, Ebrahim W, Mándi A, Kurtán T, Müller WEG, Kalscheuer R, Singab A, Lin W, Liu Z, Proksch P. Hydroquinone derivatives from the marine-derived fungus Gliomastix sp.. RSC Adv 7, 30640-30649. doi: 10.1039/C7RA04941B (2017).    
  Ancheeva E, Küppers L, Akone SH, Ebrahim W, Liu Z, Mándi A, Kurtán T, Lin W, Orfali R, Rehberg N, Kalscheuer R, Daletos G, Proksch P. Expanding the metabolic profile of the fungus Chaetomium sp. through co-culture with autoclaved Pseudomonas aeruginosa. Eur J Org Chem 2017, 3256–3264. doi:10.1002/ejoc.201700288 (2017).    
  Elshaarawy RFM, Mustafa FHA, van Geelen L, Abou-Taleb AEA, Tadros HRZ, Kalscheuer R, Janiak C. Mining marine shell wastes for polyelectrolyte chitosan anti-biofoulants: Fabrication of high-performance economic and ecofriendly anti-biofouling coatings. Carbohydr Polym 172, 352-364. doi: 10.1016/j.carbpol.2017.05.059 (2017).    
  Prados-Rosales R, Carreño L, Cheng T, Blanc C, Weinrick B, Malek A, Lowary TL, Baena A, Joe M, Bai Y, Kalscheuer R, Batista-Gonzalez A, Saavedra NA, Sampedro L, Tomás J, Anguita J, Hung SC, Tripathi A, Xu J, Glatman-Freedman A, Jacobs WR Jr, Chan J, Porcelli SA, Achkar JM, Casadevall A. Enhanced control of Mycobacterium tuberculosis extrapulmonary dissemination in mice by an arabinomannan-protein conjugate vaccine. PLoS Pathog. 13, e1006250. doi: 10.1371/journal.ppat.1006250 (2017).    
  Nkwouano V, Witkowski S, Rehberg N, Kalscheuer R, Nausch N, Mayatepek E, Jacobsen M. A novel mycobacterial in vitro infection assay identifies differences of induced macrophage apoptosis between CD4+ and CD8+ T cells. PLoS One 12, e0171817. doi: 10.1371/journal.pone.0171817 (2017).    
  Muharini R, Díaz A, Ebrahim W, Mándi A, Kurtán T, Rehberg N, Kalscheuer R, Hartmann R, Orfali RS, Lin W, Liu Z, Proksch P. Antibacterial and cytotoxic phenolic metabolites from the fruits of Amorpha fruticosa. J Nat Prod 80, 169-180. doi: 10.1021/acs.jnatprod.6b00809 (2017).    
2016      
  Korte J, Alber M, Trujillo CM, Syson K, Koliwer-Brandl H, Deenen R, Köhrer K, DeJesus MA, Hartman T, Jacobs WR Jr, Bornemann S, Ioerger TR, Ehrt S, Kalscheuer R. Trehalose-6-phosphate-mediated toxicity determines essentiality of OtsB2 in Mycobacterium tuberculosis in vitro and in mice. PLoS Pathog. 12:e1006043. doi: 10.1371/journal.ppat.1006043 (2016).    
  Liu S, Dai H, Konuklugil B, Orfali RS, Lin W, Kalscheuer R, Liu Z , Proksch P. Phenolic bisabolanes from the sponge-derived fungus Aspergillus sp.. Phytochem Lett 18, 187–191. doi: 10.1016/j.phytol.2016.10.015 (2016).    
  Daletos G, Ancheeva E, Chaidir C, Kalscheuer R, Proksch P. Antimycobacterial metabolites from marine invertebrates. Arch Pharm (Weinheim) 349:763-773. doi: 10.1002/ardp.201600128. Review. (2016).    
  Koliwer-Brandl H, Syson K, van de Weerd R, Chandra G, Appelmelk B, Alber M, Ioerger TR, Jacobs Jr. WR,  Geurtsen J, Bornemann S, Kalscheuer R. Metabolic network for the biosynthesis of intra- and extracellular α-glucans required for virulence of Mycobacterium tuberculosis. PLoS Pathog 12, e1005768. doi:10.1371/journal.ppat.1005768 (2016).    
  Steiger J, Stephan A, Inkeles MS, Realegeno S, Bruns H, Kröll P, de Castro Kroner J, Sommer A, Batinica M, Pitzler L, Kalscheuer R, Hartmann P, Plum G, Stenger S, Pellegrini M, Brachvogel B, Modlin RL, Fabri M. Imatinib triggers phagolysosome acidification and antimicrobial activity against Mycobacterium bovis Bacille Calmette-Guérin in glucocorticoid-treated human macrophages. J Immunol 197, 222-232. doi: 10.4049/jimmunol.1502407 (2016).    
  Rashid AM, Batey SF, Syson K, Koliwer-Brandl H, Miah F, Barclay JE, Findlay KC, Nartowski KP, Khimyak YZ, Kalscheuer R, Bornemann S. The assembly of α-glucan by GlgE and GlgB in mycobacteria and streptomycetes. Biochemistry 55, 3270-3284. doi: 10.1021/acs.biochem.6b00209 (2016).    
  Ebrahim W, El-Neketi M, Lewald LI, Orfali RS, Lin W, Rehberg N, Kalscheuer R, Daletos G, Proksch P. Metabolites from the fungal endophyte Aspergillus austroafricanus in axenic culture and in fungal-bacterial mixed cultures. J Nat Prod 79, 914-922 (2016).    
  Famulla K, Sass P, Malik I, Akopian T, Kandror O, Alber M, Hinzen B, Ruebsamen-Schaeff H, Kalscheuer R, Goldberg AL, Brotz-Oesterhelt H. Acyldepsipeptide antibiotics kill mycobacteria by preventing the physiological functions of the ClpP1P2 protease. Mol Microbiol 101, 194-209. doi: 10.1111/mmi.13362 (2016).    
2015      
  Ates LS, Ummels R, Commandeur S, van de Weerd R, Sparrius M, Weerdenburg E, Alber M, Kalscheuer R, Piersma SR, Abdallah AM, Abd El Ghany M, Abdel-Haleem AM, Pain A, Jimenez CR, Bitter W, Houben EN. Essential role of the ESX-5 secretion system in outer membrane permeability of pathogenic mycobacteria. PLoS Genet 11, e1005190 (2015).    
  Daletos G, Kalscheuer R, Koliwer-Brandl H, Hartmann R, de Voogd NJ, Wray V, Lin W, Proksch P. Callyaerins from the marine sponge Callyspongia aerizusa: Cyclic peptides with antitubercular activity. J Nat Prod 78, 1910-1925 (2015).    
       
2014      
  Kalscheuer R, Koliwer-Brandl H. Genetics of mycobacterial trehalose metabolism. Microbiol Spectr 2 (2014).    
  Urbanek BL, Wing DC, Haislop KS, Hamel CJ, Kalscheuer R, Woodruff PJ, Swarts BM. Chemoenzymatic synthesis of trehalose analogues: rapid access to chemical probes for investigating mycobacteria. Chembiochem 15, 2066-2070 (2014).    
       
2013      
  Miah F, Koliwer-Brandl H, Rejzek M, Field RA, Kalscheuer R, Bornemann S. Flux through trehalose synthase flows from trehalose to the alpha anomer of maltose in mycobacteria. Chem Biol 20, 487-493 (2013).    

 

Leiba J, Syson K, Baronian G, Zanella-Cleon I, Kalscheuer R, Kremer L, Bornemann S, Molle V. Mycobacterium tuberculosis maltosyltransferase GlgE, a genetically validated antituberculosis target, is negatively regulated by Ser/Thr phosphorylation. J Biol Chem 288, 16546-16556 (2013).    
       
2012      
  Swarts BM, Holsclaw CM, Jewett JC, Alber M, Fox DM, Siegrist MS, Leary JA, Kalscheuer R, Bertozzi CR. Probing the mycobacterial trehalome with bioorthogonal chemistry. J Am Chem Soc 134, 16123-16126 (2012).    
       
2011      
  Prados-Rosales R, Baena A, Martinez LR, Luque-Garcia J, Kalscheuer R, Veeraraghavan U, Camara C, Nosanchuk JD, Besra GS, Chen B, Jimenez J, Glatman-Freedman A, Jacobs WR, Jr., Porcelli SA, Casadevall A. Mycobacteria release active membrane vesicles that modulate immune responses in a TLR2-dependent manner in mice. J Clin Invest 121, 1471-1483 (2011).    
       
2010      
  Kalscheuer R, Syson K, Veeraraghavan U, Weinrick B, Biermann KE, Liu Z, Sacchettini JC, Besra G, Bornemann S, Jacobs WR, Jr. Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway. Nat Chem Biol 6, 376-384 (2010).    
  Kalscheuer R, Jacobs WR, Jr. The significance of GlgE as a new target for tuberculosis. Drug News Perspect 23, 619-624 (2010).    
  Manilla-Perez E, Reers C, Baumgart M, Hetzler S, Reichelt R, Malkus U, Kalscheuer R, Waltermann M, Steinbuchel A. Analysis of lipid export in hydrocarbonoclastic bacteria of the genus Alcanivorax: identification of lipid export-negative mutants of Alcanivorax borkumensis SK2 and Alcanivorax jadensis T9. J Bacteriol 192, 643-656 (2010).    
  Manilla-Perez E, Lange AB, Hetzler S, Waltermann M, Kalscheuer R, Steinbuchel A. Isolation and characterization of a mutant of the marine bacterium Alcanivorax borkumensis SK2 defective in lipid biosynthesis. Appl Environ Microbiol 76, 2884-2894 (2010).    
  Kalscheuer R, Weinrick B, Veeraraghavan U, Besra GS, Jacobs WR, Jr. Trehalose-recycling ABC transporter LpqY-SugA-SugB-SugC is essential for virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 107, 21761-21766 (2010).    
       
2009      
  Kaddor C, Biermann K, Kalscheuer R, Steinbuchel A. Analysis of neutral lipid biosynthesis in Streptomyces avermitilis MA-4680 and characterization of an acyltransferase involved herein. Appl Microbiol Biotechnol 84, 143-155 (2009).    
  Yam KC, D'Angelo I, Kalscheuer R, Zhu H, Wang JX, Snieckus V, Ly LH, Converse PJ, Jacobs WR, Jr., Strynadka N, Eltis LD. Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis. PLoS Pathog 5, e1000344 (2009).    
  Capyk JK, Kalscheuer R, Stewart GR, Liu J, Kwon H, Zhao R, Okamoto S, Jacobs WR, Jr., Eltis LD, Mohn WW. Mycobacterial cytochrome p450 125 (cyp125) catalyzes the terminal hydroxylation of c27 steroids. J Biol Chem 284, 35534-35542 (2009).    
       
2008      
  Alvarez AF, Alvarez HM, Kalscheuer R, Waltermann M, Steinbuchel A. Cloning and characterization of a gene involved in triacylglycerol biosynthesis and identification of additional homologous genes in the oleaginous bacterium Rhodococcus opacus PD630. Microbiology 154, 2327-2335 (2008).    
       
2007      
  Kalscheuer R, Stoveken T, Malkus U, Reichelt R, Golyshin PN, Sabirova JS, Ferrer M, Timmis KN, Steinbuchel A. Analysis of storage lipid accumulation in Alcanivorax borkumensis: Evidence for alternative triacylglycerol biosynthesis routes in bacteria. J Bacteriol 189, 918-928 (2007).    
       
2006      
  Sabirova JS, Ferrer M, Lunsdorf H, Wray V, Kalscheuer R, Steinbuchel A, Timmis KN, Golyshin PN. Mutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2. J Bacteriol 188, 8452-8459 (2006).    
  Kalscheuer R, Stoveken T, Luftmann H, Malkus U, Reichelt R, Steinbuchel A. Neutral lipid biosynthesis in engineered Escherichia coli: jojoba oil-like wax esters and fatty acid butyl esters. Appl Environ Microbiol 72, 1373-1379 (2006).    
  Kalscheuer R, Stolting T, Steinbuchel A. Microdiesel: Escherichia coli engineered for fuel production. Microbiology 152, 2529-2536 (2006).    
       
2005      
  Waltermann M, Hinz A, Robenek H, Troyer D, Reichelt R, Malkus U, Galla HJ, Kalscheuer R, Stoveken T, von Landenberg P, Steinbuchel A. Mechanism of lipid-body formation in prokaryotes: how bacteria fatten up. Mol Microbiol 55, 750-763 (2005).    
  Uthoff S, Stoveken T, Weber N, Vosmann K, Klein E, Kalscheuer R, Steinbuchel A. Thio wax ester biosynthesis utilizing the unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase of Acinetobacter sp. strain ADP1. Appl Environ Microbiol 71, 790-796 (2005).    
  Stoveken T, Kalscheuer R, Malkus U, Reichelt R, Steinbuchel A. The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase. J Bacteriol 187, 1369-1376 (2005).    
       
2004      
  Kalscheuer R, Luftmann H, Steinbuchel A. Synthesis of novel lipids in Saccharomyces cerevisiae by heterologous expression of an unspecific bacterial acyltransferase. Appl Environ Microbiol 70, 7119-7125 (2004).    
       
2003      
  Kalscheuer R, Steinbuchel A. A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1. J Biol Chem 278, 8075-8082 (2003).    
  Arenskotter M, Baumeister D, Kalscheuer R, Steinbuchel A. Identification and application of plasmids suitable for transfer of foreign DNA to members of the genus Gordonia. Appl Environ Microbiol 69, 4971-4974 (2003).    
       
2001      
  Kalscheuer R, Waltermann M, Alvarez M, Steinbuchel A. Preparative isolation of lipid inclusions from Rhodococcus opacus and Rhodococcus ruber and identification of granule-associated proteins. Arch Microbiol 177, 20-28 (2001).    
       
2000      
  Alvarez HM, Kalscheuer R, Steinbuchel A. Accumulation and mobilization of storage lipids by Rhodococcus opacus PD630 and Rhodococcus ruber NCIMB 40126. Appl Microbiol Biotechnol 54, 218-223 (2000).    
  Waltermann M, Luftmann H, Baumeister D, Kalscheuer R, Steinbuchel A. Rhodococcus opacus strain PD630 as a new source of high-value single-cell oil? Isolation and characterization of triacylglycerols and other storage lipids. Microbiology 146 ( Pt 5), 1143-1149 (2000).    
       
1999      
  Spiekermann P, Rehm BH, Kalscheuer R, Baumeister D, Steinbuchel A. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 171, 73-80 (1999).    
  Kalscheuer R, Arenskotter M, Steinbuchel A. Establishment of a gene transfer system for Rhodococcus opacus PD630 based on electroporation and its application for recombinant biosynthesis of poly(3-hydroxyalkanoic acids). Appl Microbiol Biotechnol 52, 508-515 (1999).    
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